Helicobacter pylori amoxicillin heteroresistance due to point mutations in PBP-1A in isogenic isolates

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Helicobacter pylori amoxicillin heteroresistance due to point mutations in PBP-1A in isogenic isolates.

OBJECTIVES To investigate the Helicobacter pylori amoxicillin resistance rate, the occurrence of heteroresistance, and their related molecular mechanisms. METHODS Eighty-seven H. pylori-positive patients were included: 45/87 with single biopsy and 42/87 with multiple biopsies. MICs were determined, and sequencing analysis of pbp1A gene and the variable regions of seven hop porins was performe...

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A change in PBP1 is involved in amoxicillin resistance of clinical isolates of Helicobacter pylori.

Reports on the isolation of amoxicillin-resistant Helicobacter pylori are increasing worldwide, which may cause serious problems in eradication therapy. To elucidate the mechanism of amoxicillin resistance of H. pylori, penicillin-binding proteins (PBPs) of amoxicillin-resistant strains isolated in Korea were analysed. Three PBPs (66, 63 and 60 kDa) were identified in both amoxicillin-resistant...

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Resistance Rates to Various Antimicrobial Agents of Helicobacter pylori Isolates in Eastern Turkey

The aim of this study was to determine the resistance rates of Helicobacter pylori (H. pylori) to various antimicrobial agents. The agar disk diffusion method (Kirby Bauer) was used to determine the sensitivity of H. pylori isolates to various antimicrobials. Of the 61 H. pylori isolatestested, no isolates was resistant to amoxycillin and tetracycline. The resistance rates were 42.6% for metron...

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Sensitivity of amoxicillin-resistant Helicobacter pylori to other penicillins.

The sensitivities to penicillins and to a penicillin and beta-lactamase inhibitor combination agent were determined for Helicobacter pylori strains that were sensitive, moderately resistant, or highly resistant to amoxicillin. All strains were resistant to nafcillin and oxacillin. Moderately resistant strains showed an intermediate zone of inhibition to ticarcillin, mezlocillin, piperacillin, a...

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Detection of A2142C, A2142G, and A2143G Mutations in 23s rRNA Gene Conferring Resistance to Clarithromycin among Helicobacter pylori Isolates in Kerman, Iran

Background: Clarithromycin resistance in Helicbacter pylori has been found to be associated with point mutations in 23s rRNA gene leads to reduced affinity of the antibiotic to its ribosomal target or changing the site of methylation. The aim of this study was to determine the most important point mutations in 23s rRNA gene in H. pylori that are closely related to clarith-romycin resistance amo...

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ژورنال

عنوان ژورنال: Journal of Antimicrobial Chemotherapy

سال: 2008

ISSN: 0305-7453,1460-2091

DOI: 10.1093/jac/dkm504